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Microbiology / Infection Panel Molecular Diagnostic Products

MICROBIOLOGY

INFECTION PANEL

HIV Qualitative Rt-PCR kit HIV Quantitive Rt-PCR kit

The HIV-1 Rt-PCR Qualitative Kit is a real-time reverse tran- The HIV-1 Rt-PCR Quantitative Kit is a real-time reverse
scription polymerase chain reaction (RT-qPCR) molecular transcription polymerase chain reaction (RT-qPCR)
diagnostic kit developed for the qualitative determination molecular diagnostic kit developed for the quantitative
of the presence of HIV-1 RNA in the plasma of AIDS pa- determination of the HIV-1 RNA load in the plasma of
tients in an in vitro environment. The kit includes all the AIDS (acquired immunodeficiency syndrome) patients
necessary components to detect HIV-1 RNA in the plasma in an in vitro environment. The kit is a quantitative test
of patients with acquired immunodeficiency syndrome that, along with clinical findings and other laboratory
(AIDS). Within the kit, there are primers that amplify a data, assists in monitoring the prognosis of the disease
121-base region within the long terminal repeat (LTR) of and the effectiveness of antiviral treatment.
the HIV-1 genome, along with a TaqMan probe labeled
with a fluorophore dye and suppressor for detection. The kit includes primers that amplify a 121-base region
within the long terminal repeat (LTR) of the HIV-1 genome,
In the presence of HIV-1 RNA in the sample, during the along with a TaqMan probe labeled with a fluorophore
amplification program applied after the reverse transcrip- dye and suppressor for detection. Additionally, four
tion stage, the target region is amplified using forward quantification standards (KS1-KS4) are included in the
and reverse primers. During the annealing stage of the kit. In the presence of HIV-1 RNA in the sample, during
amplification program, TaqMan probes bind to the tar- the amplification program applied after the reverse
get region. In the extension stage, the DNA polymerase transcription stage, the target region is amplified using
enzyme cleaves the probe from its 5’ end, freeing it from forward and reverse primers.
the suppressor at the 3’ end and emitting fluorescence.
The resulting fluorescent signals are collected throughout During the annealing stage of the amplification program,
the amplification process and reported in the form of TaqMan probes bind to the target region. In the extension
amplification graphs. stage, the DNA polymerase enzyme cleaves the probe from
its 5’ end, freeing it from the suppressor at the 3’ end and
emitting fluorescence. The resulting fluorescent signals
are collected throughout the amplification process and
reported in the form of amplification graphs.

The HIV-1 RNA load in positive samples is determined
using data from copy/mL corresponding to quantification
standards (KS1-KS4) and their corresponding Ct values,
generated from a standard curve created in each study.

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