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Microbiology / Infection Panel Molecular Diagnostic Products

MICROBIOLOGY

INFECTION PANEL

Hepatitis B Qualitative Rt-PCR kit Hepatitis B Quantitive Rt-PCR kit

The DiaRD-HBV Rt-PCR Qualitative Kit is a real-time poly- The DiaRD-HBV Rt-PCR Quantitative Kit is a real-time
merase chain reaction (Rt-PCR) molecular diagnostic kit polymerase chain reaction (Rt-PCR) molecular diagnostic
developed to investigate the presence of HBV DNA in the kit developed for the quantitative determination of HBV
plasma of hepatitis B patients in an in vitro environment. DNA load in the plasma of hepatitis B patients in an in
The kit includes primers that amplify a 90-base pair region vitro environment. The kit includes primers that amplify
within the HBV S gene, along with fluorophore dye and a a 90-base pair region within the HBV S gene, along with
TaqMan probe labeled with a suppressor for detection. In fluorophore dye and a TaqMan probe labeled with a sup-
the presence of HBV DNA in the sample, the target region pressor for detection. In the presence of HBV DNA in the
is amplified during the applied amplification program sample, the target region is amplified during the applied
using forward and reverse primers. amplification program using forward and reverse primers.

During the annealing stage of the amplification program, During the annealing stage of the amplification program,
TaqMan probes bind to the target region. In the extension TaqMan probes bind to the target region. In the extension
stage, the DNA polymerase enzyme cleaves the probe from stage, the DNA polymerase enzyme cleaves the probe from
its 5’ end, freeing it from the suppressor at the 3’ end and its 5’ end, freeing it from the suppressor at the 3’ end and
emitting fluorescence. The resulting fluorescent signals emitting fluorescence. The resulting fluorescent signals
are collected throughout the amplification process and are collected throughout the amplification process and
reported in the form of amplification graphs. reported in the form of amplification graphs.

The HBV DNA load in positive samples is determined
using data from IU/mL corresponding to quantification
standards (KS1-KS5) and their corresponding Ct values,
generated from a standard curve created in each study.

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