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Microbiology / Infection Panel
Molecular Diagnostic Products
MICROBIOLOGY

INFECTION PANEL

Hepatitis C Qualitative Rt-PCR kit Hepatitis C Quantitive Rt-PCR kit

The Hepatitis C virus primarily causes Hepatitis C disease, The Hepatitis C virus primarily causes Hepatitis C disease,
affecting the liver through transmission, mainly via blood, affecting the liver through transmission, mainly via blood,
as well as through sexual contact and transmission from as well as through sexual contact and transmission from
mother to child via the placenta. The DiaRD-HCV RT-qPCR mother to child via the placenta. The DiaRD-HCV RT-qPCR
Qualitative Kit is a real-time reverse transcription poly- Quantitative Kit is a real-time reverse transcription poly-
merase chain reaction (RT-qPCR) diagnostic kit developed merase chain reaction (RT-qPCR) diagnostic kit developed
for the purpose of detecting the presence of HCV RNA in for the quantitative determination of HCV RNA load in
the plasma of Hepatitis C patients. The kit includes primers the plasma of Hepatitis C patients in an in vitro environ-
that amplify a 105-base region within the 5’ untranslated ment. The kit includes primers that amplify a 105-base
region (UTR) of the HCV genome, along with a TaqMan region within the 5’ untranslated region (UTR) of the HCV
probe for detection. genome, along with a TaqMan probe for detection and
quantification standards used to determine RNA quantity.
In the presence of HCV RNA in the sample, during the
amplification program applied after the reverse transcrip- In the presence of HCV RNA in the sample, during the
tion stage, the target region is amplified using forward amplification program applied after the reverse transcrip-
and reverse primers. During the annealing stage of the tion stage, the target region is amplified using forward
amplification program, TaqMan probes bind to the tar- and reverse primers. During the annealing stage of the
get region. In the extension stage, the DNA polymerase amplification program, TaqMan probes bind to the tar-
enzyme cleaves the probe from its 5’ end, freeing it from get region. In the extension stage, the DNA polymerase
the suppressor at the 3’ end and emitting fluorescence. enzyme cleaves the probe from its 5’ end, freeing it from
The resulting fluorescent signals are collected throughout the suppressor at the 3’ end and emitting fluorescence.
the amplification process and reported in the form of The resulting fluorescent signals are collected throughout
amplification graphs. the amplification process and reported in the form of
amplification graphs.

The HCV RNA load in positive samples is determined
using data from IU/mL corresponding to quantification
standards (KS1-KS4) and their corresponding Ct values,
generated from a standard curve created in each study.

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