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Species Determination Panel
                                                              Molecular Genetics Diagnostic Products
                                                          SPECIES DETERMINATION

                                                                                              PANEL








           3026-100  Staphylococcus aureus RT-PCR Kit





           Real-time  PCR  Kit  for  Staphylococcus aureus has   S. aureus can cause a variety of different infections, from
           been developed for in vitro quantification of S. aureus   mild skin infections such as impetigo to invasive diseases
           genomes. The kit has been designed to have the broad    and toxic-medi ated diseases. Production of exo toxins
           estpossible  detection profile while remaining  specific   known as pyrogenic toxin superantigens in some strains
           to the S.  aureus genome. The primer sequences in this   of S. aureus can cause toxic shock syn drome. Realtime
           kit have 100% homology with a wide range of s. aureus   PCR is the fastest and most reliable method of per forming
           sequences as a result of extensive  bioinformatics  analysis.   an accurate s. aureus de tection.
           The chromosomal  gene femA is used to detect S. aureus.

           S. aureus is a Gram-positive bacterium from the Staphy-
           lococcaceae family. The genome of this species is about
           2.8 Mb in length. Virulence factors for S. aureus are
           encoded  by  phages,  pathogenicity islands, staphylo-
           coccal cassette chro mosome, and plasmids  that  contain
           genes for antibiotic resistance, such as those found in
           Methicillin-Resistant  S. aureus (MRSA). S. aureus can be
           found on the skin and mucous  membranes  of healthy
           individuals, but erosion of these barriers  allows  bac-
           teria to enter  the wound, colonize and cause infection.
           Since this bacterium lives on the skin, it can easily be
           transmitted between indi viduals  and  is usually  seen in
           hospi tal-acquired infections.


























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