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Company Profıle
RESERACH AND DEVELOPMENT DEPARTMENT
SERVICES

PCR (Polymerase Chain Reaction) Analyses Sequencing Service RESERACH AND DEVELOPMENT DEPARTMENT

Within the R&D unit of Diagen Biotechnology, genetic Sanger Sequencing Analyses
research is conducted using PCR systems, specializing
in mutation analyses, molecular diagnosis, and species Diagen Biotechnology provides services for your projects
typing with an expert team. In addition to routine ser- with DNA sequencing analyses. Using Sanger sequencing
vices, laboratory support is provided for projects. Our systems, sequence analyses of samples, nucleic acids, or
laboratory and bioinformatics unit, staffed with experts, PCR products are conducted, and results are delivered
work to provide reliable services by listening to individual as quickly as possible.
needs and preferences.
Genetic material sequencing processes are carried out
In our laboratory, we use Roche LightCycler 480 II, ABI7500 using the ABI Prism Genetic Analyzer.
Fast, ESCO 96, and Qiagen Rotor Gene Q devices, capable
of providing accurate measurements for conventional PCR Serological Analyses
(ABI Veriti and Aeris) and real-time PCR studies.
Our goal is to provide personalized service by meeting
Our goal is to provide personalized services by meeting individual requests such as reporting format, turnaround
individual requests such as reporting format, turnaround time, and plan changes based on the methods used in
time, and plan changes based on the methods used in the studies.
the studies.
In ELISA diagnostic and research kit applications:
Key Focus Areas in PCR Studies: - Relevant applications for the kits

- Mutation Analyses - Relevant analyses after application

- Allele Detection
- Reporting is carried out
- Hydrolysis and Hybridization Probe-based QPCR
Design of Primers and Probe Sets
- Non-Specific qPCR (EvaGreen, SybrGreen, and Interca-
lating Dyes) Within the scope of your studies, primer and probe sets
are designed according to the experimental purpose.
- Gene Expression Analyses In the design consultancy, genetic information of the

desired species is examined, and designs are created
- Telomere Length Analyses
for conventional PCR, dsDNA-binding dyes, hydrolysis,
- miRNA Expression Analyses and hybridization probes.

- miRNA Profiling
- miRNA Pathway Analyses

- cDNA Synthesis

- Specific Primer and Probe Design
- Methylation Analyses

- Optimization Studies

- Specificity and Sensitivity Studies
- Primer and Probe Optimizations

- Device Optimization www.diagen.com.tr

- Internal Laboratory Inspection
- External Laboratory Inspection

info@diagen.com.tr 67