Page 64 - DIAGEN-EN

Recombinant Products

PROTEIN AND ENZYME
APPLICATIONS Species Determination Panel

DiaTaq DNA Polimerase DiaTaq DNA Polimerase
2X Master Mix
Taq DNA Polymerase is a thermostable enzyme derived from the thermo-
philic bacterium *Thermus aquaticus*. The enzyme is in recombinant form DiaTaq DNA Polymerase 2x Master Mix is a ready-touse 2x reaction
expressed in *E. coli*. It can withstand repeated heating up to 95°C without mixture containing DiaTaq DNA polymerase, dNTPs, and magnesium
chloride. For each reaction, half of the total reaction volume is made
significant loss of activity. The enzyme has an approximate size of 94 kDa on up of the 2x Master Mix. You only need to add primers, DNA, and
SDS-PAGE. It exhibits 5’- >3’ DNA polymerase activity and 5’->3’ exonuclease dH2O to complete the total volume for each reaction. The Taq DNA
activity. Each batch of Taq DNA Polymerase is tested for PCR amplification Polymerase 2x Master Mix offers several advantages. It significantly
and double-stranded sequencing. The enzyme is supplied at 5 units/mL and reduces setup time and eliminates the risk of contaminating compo-
comes with an optimized 10x reaction buffer. nent stocks. By reducing reagent handling steps, it allows for better
reproducibility of experiments. Standard tests can be set up with
Unit Description confidence, ensuring consistent results every time.
Taq DNA Polymerase 2x Master Mix consists of the following
One unit incorporates 10 nmol of total deoxyribonucleotide triphosphates components:
into acidprecipitable DNA within 30 minutes at 74°C.
• Tris-HCl pH 8.4, 3 mM MgCl2,
Reactives
• %0,1 Triton X-100,
·**Taq DNA Polymerase Composition:**
• 0.4 mM dNTP
- 20 mM Tris-HCl, pH 8.0
• DiaTaq DNA polimerase
- 5 units/mL Taq DNA Polymerase
• Stabilizers
- 100 mM KCl
Quality Control
- 0.1 mM EDTA
Taq DNA Polymerase has been tested for contamination activities and
- 1 mM DTT
shown to have no endonuclease activity, nicking activity, or exonucle-
- Stabilizers ase activity.
- 50% Glycerol Recombinant Protein
**10x PCR Buffer Composition:** • Codon Optimization

- 100 mM Tris-HCl, pH 8.3 • Plasmid design
- 500 mM KCl • Preparation of Compotent Cells
- 15 mM MgCl2 • Transformation procedings
Taq Polimerase PCR studies • Recombinant Protein production and purification

• SDS PAGE service
ARMORED RNA

In RNA-based PCR studies, we offer more stable kits by protecting
RNAs to be used as controls with coat proteins.
Enzyme and Mastermix Production

• Taq DNA Polimerase Enzyme
• Reverse Transcriptase Enzyme
• DNA Polimerase I
Product List • Hotstart Taq DNA Polimerase One

4001-T1 DiaTaq DNA Polimerase /100U (20uL) • Step Mastermix
• 2x Taq Mastermix
4001-T5 DiaTaq DNA Polimerase /500U (100uL)
ELISA
4001-T10 DiaTaq DNA Polimerase /1000U (200uL)
• Research Kits training and application services
4001-T50 DiaTaq DNA Polimerase /5000U (1000uL) www.diagen.com.tr
• Development of ELISA Research and Diagnostic Kits
4002-MM DiaTaq 2x Master Mix
Lateral Flow

• Kit developments
PRODUCT CATALOGUE 64